Flp-in cho
WebNov 8, 2013 · The Flp-In CHO cell line contains a single integrated FRT site and stably expresses the lacZ-Zeocin fusion gene. Flp-In CHO cells were cultured in Ham’s F12 medium (Nissui, Tokyo, Japan) containing 100 U/ml of penicillin (Meiji Seika, Tokyo, Japan), 100 μg/ml of streptomycin (Meiji Seika), 100 μg/ml of Zeocin (Invitrogen) and 10% fetal ... WebFlp-In™Host Cell Lines For your convenience, Invitrogen has available several mammalian Flp-In™host cell lines that stably express the lacZ-Zeocin™fusion gene from pFRT/lacZeo or pFRT/lacZeo2. Each cell line contains a single integrated FRT site as confirmed by Southern blot analysis.
Flp-in cho
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WebA single gene copy of shRNA-2 also decreased the translational level of DHFR by 80% in Flp-In CHO cells. shRNA-2 was then incorporated into a plasmid vector expressing human erythropoietin (EPO) and an exogenous DHFR to develop … WebLike Cre, FLP is a tyrosine recombinase, but it originates from the yeast Saccharomyces cerevisiae, rather than from bacteriophage. In FLP-FRT, FLP is analogous to cre and FRT to loxP – FLP recognizes the FRT sites, and catalyzes recombination between them.
WebThe Flp-In cell lines should be maintained in medium containing Zeocin. For more information about pFRT/lacZeo and pcDNA5/FRT/CAT, please refer to the Flp-In System … WebDec 1, 2024 · Flp-In-CHO cells (Life Technologies) were maintained in F12 medium (Gibco) supplemented with 10% heat-inactivated fetal bovine serum (FBS; Gibco) at 37 °C in a humidified incubator containing 5% CO 2. The cells were transfected with 1 μg plasmid (pDF-λ1:pOG44 was 1:9) using Lipo 2000 (Life Technologies) according to the …
WebFeb 11, 2024 · Results: A range of sialyltransferase from mammals, birds and fish were stably expressed in CHO Flp-In cells. The stable cell lines expressing ST6Gal1 modify the glycans on the surface of the CHO cells as detected by fluorescently labelled lectin microscopy. The catalytic domains, as isolated by Ni Sepharose from culture media, … WebFlp-In™ 细胞系设计用于快速生成稳定细胞系以从 Flp-In™ 表达载体表达关注的蛋白。这些细胞在转录活性基因组基因座处包含一个稳定整合的 FRT 位点。Flp-In™ 表达载体的靶向 …
WebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and multidrug resistance-associated protein 2(MRP2).
WebA, assay for the evaluation of the interaction between cells and surface-bound collectins. Step 1: incubation of AF 488-conjugated bioparticles with collectins. Step 2: incubation of … fl airport mapWebOct 1, 2024 · Objective To construct a double transfected Flp-InTM CHO cell line stably expressing both cytochrome P450 family 2 subfamily A member 13(CYP2A13) and … flairprint walsallWebMar 5, 2024 · Idiopathic Basal Ganglia Calcification (IBGC) is a rare neuropsychiatric illness also known as Fahr's disease or Primary Familial Brain Calcification (PFBC). IBGC is caused by SLC20A2 variants, which encodes the inorganic phosphate (Pi) transporter PiT-2, a transmembrane protein associated with Pi homeostasis. canopy netting for a bedWebAug 20, 2024 · The Flp-In system integrates a vector into cell lines at a specific genomic locus, but has not been used for integration of more than one gene. ... X. et al. … flair plane crashWebNov 11, 2013 · Flip-In CHO cells (FCHO) stably transfected by plasmid DNA were stained by PE-conjugated mouse-anti-human kappa chain antibodies (PE-Kappa) and then analyzed by FACS. ... Invitrogen (2006) Flp-In system for generating stable mammalian expression cell lines by Flp recombinase-mediated integration. Instruction Manual. Carlsbad, CA. 22. fl airporthelp picking car rentalsflair plug ins for photoshopWebJan 15, 2024 · We also established CHO cells expressing the WT protein using the Flp-In system as previously described (thereafter called Flp-In CHO-WT cells) [6]. The pcDNA5/FRT-PiT-2-flag in GFP vectors were obtained by inserting GFP into Loop7 of the SLC20A2 (WT, T115 M, and R467X) using in-fusion cloning system (Takara Bio Inc.; … flair realty