D10.g4.1 mouse helper t cells
WebJun 15, 1987 · After activation with antigen or mitogen, D10.G4.1, an antigen-specific cloned T helper cell that has recently been shown to produce IL 4 but not IL 2, secretes two … WebNov 1, 1989 · We describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1β or α in the …
D10.g4.1 mouse helper t cells
Did you know?
WebD10 G4 1 T Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol … WebApr 13, 2024 · Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 beta /IL …
WebJun 22, 2024 · Briefly, D10.G4.1 cells (2 x 10 6 cells/well) were dispensed into anti-CD3 mAb coated 6-well plates, with or without varying concentrations of NK-4, and incubated for 6 h at 37°C. Total RNA was extracted from D10.G4.1 cells using an RNeasy Mini kit (QIAGEN, Tokyo, Japan) and DNase (QIAGEN) according to the manufacturer’s … WebThe biological activity of recombinant rat IL-1α was measured by its ability to stimulate cell proliferation using a mouse helper T cell line, D10.G4.1. Safety Information. Storage Class Code. 13 - Non Combustible Solids. WGK. WGK 3. Flash Point(F) Not applicable. Flash Point(C) Not applicable.
WebMouse: Full name is D10G4.1. This is a Th2 helper cell line, derived from AKR/J (H-2K) mice, specific for egg white conalbumin. Tissues: Lymphocyte: Phenotype: Suspension: … WebJun 22, 2024 · In our study, however, T-bet mRNA expression was not examined in Fig 5, since T-bet is not expressed in D10.G4.1 cells . NK-4 had little effect on IFN-γ …
WebATCC mouse d10 g4 1 t helper 2 cell Mouse D10 G4 1 T Helper 2 Cell, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed …
phil higgins jacobsWebNov 26, 2024 · Recombinant MouseIL‑1 beta /IL‑1F2 (Catalog # 401-ML) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL‑1 beta /IL‑1F2 (50 pg/mL) is neutralized (green line) by … phil hiebert lawyerWebNov 4, 2024 · DL0410 has been shown to reverse cognitive impairments in AD animal models, including an Aβ 1–42-induced amnesia mouse model, an APP/PS1 transgenic … phil higham rsmWebAug 1, 1989 · IL-6 also affects murine and human T cells and thymocytes. Our study was designed to analyze the effects of IL-1 and IL-6 in providing a second signal in T cell activation in the D10.G4.1 assay. Highly purified human rIL-6 was tested. rIL-6 had modest but detectable activity in the D10.G4.1 assay. phil higginson dwpWebApr 13, 2024 · D10.G4.1 mouse helper T cells in ... costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells (TFH) and germinal centers (35, 9, 10). In human but not in mouse, B7H2 additionally binds to CD28 and CTLA4 , and its interaction with CD28 can costimulate both human and mouse naïve ... phil hieterWebED 50 10.0 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, ... keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte ... phil hicks tulaneWebRecombinant Mouse IL-1 alpha /IL-1F1 (Catalog # 400-ML) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED 50 for this effect is 3-7 pg/mL. SDS … phil higgins wmas