WebApr 7, 2024 · For GST pull-down, 5 µg of each GST proteins conjugated to 20 µl of Glutathione Sepharose slurry were incubated on wheel for 1 h with cell lysate in buffer IP150 supplemented with PI, NaF and β-glycerophosphate, or with 5 µg of purified recombinant proteins in IP150 supplemented with PI. Beads were washed 3 times with … WebUse for high capacity purification of Glutathione S-Transferase (GST) tagged fusion proteins. Cytiva Life Sciences™ Glutathione Sepharose™ 4B Media requires only a …
Glutathione Sepharose 4 Fast Flow GST-tagged protein …
WebApr 9, 2024 · By following simple protein engineering steps, recombinant proteins with promising applications in the field of drug delivery can be assembled in the form of functional materials of increasing complexity, either as nanoparticles or nanoparticle-leaking secretory microparticles. Among the suitable strategies for protein assembly, the use of histidine … WebGlutathione Sepharose selection guide ... Cytiva; Gel filtration liquid. Choose a samples volume of < 0.5% of the procession volume for media with average particle sizes in an ranging is 10-15 µm and < 5% of the volume used browse for average particle size in the range of 30-100 µm. girl thing girls on top
Glutathione Sepharose 4 Fast Flow, 25 mL Cytiva
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